In Disney Princess: My Fairytale Adventure, players will take on the role of an apprentice to the Fairy Godmother and will accidentally set off a series of events that cause friendly Sprites to transform into mischievous Imps. The evil Imps make their way into the worlds of Cinderella, Rapunzel, Ariel, Belle and Tiana, and players must pursue the Imps into each Princess kingdom to prevent them from causing any harm. With the use of their magic wand, they will need to turn the Imps back into their original Sprite form, and return them to their rightful home in the Magical Kingdom.

Each of the Princess worlds will allow players to explore and interact with familiar Disney characters such as Lumiere, Pascal, Flounder, Gus and other fan favorites who will help guide them through missions and progress though the game. Quests and mini-games, including platforming, hide and seek, and puzzles, are designed to test the skills of the youngest players and challenge older players alike.

Players can also customize their avatar and room in the Fairy Godmothers Castle, utilizing gems that are earned throughout the game to trade for items in the in-game Wishing Well store. Outfits, accessories and tiaras can be purchased to dress up avatars, creating hundreds of different combinations. Special items like floor patterns, wallpaper and furniture can be used to personalize and build out each players room in the Castle.