Set in the Matrix film universe, The Matrix: Path of Neo will enable players to actually play as Neo, the central character, and relive his most important and memorable scenarios from the complete film trilogy.. Throughout the game the path the player takes to resolve each scenario and the resulting consequences will be scripted and directed by the Wachowski Brothers.

The Matrix: Path of Neo will incorporate footage from all three feature films, as well as The Animatrix. The game includes several sequences of film-quality live-action, full-motion footage (FMV), as well as Hollywood-quality effect sequences. Throughout the game players will use martial arts, medieval weapons and guns (lots of guns) to take on a variety of enemies, such as heavily armed Agents of the Matrix, including Agent Smith and Exiles in the employ of the Merovingian.